The incident happened shortly after 7pm last night when two of the horses, which were being corralled into a trailer, bolted into the road and were in collision with a Toyota Previa.
The animals were too severely injured to be treated and were put down at the scene by a vet.
The driver of the vehicle, a 41-year-old woman from Farnham, Surrey and four passengers, a seven-year-old boy, two 10-year-old boys and a four-year-old girl, were shaken but uninjured.
The remaining four horses also escaped and ran along the southbound carriageway to London Road in Holybourne, where they were rounded up in the nearby theatre car park and returned to a secured field by agents of the owners.
The road was re-opened shortly after 9pm.
Can you help?
Any witnesses to the incident are asked to contact PC Phillipa Churcher-Brown on 101 or the Crimestoppers charity anonymously on 0800 555 111. Mini-Com users can call 01962 875000. From outside Hampshire and the Isle of Wight, please call 0845 045 45 45. To contact an officer please use our Contact an Officer Form
Ends –
Hayley Court
01962 871063 2190/141011 44110436177